● Optofluidic leakage assay

From: Measuring Thousands of Single-Vesicle Leakage Events Reveals the Mode of Action of Antimicrobial Peptides

● Optofluidic leakage assay

From: Measuring Thousands of Single-Vesicle Leakage Events Reveals the Mode of Action of Antimicrobial Peptides

● Scanning electron microscope (SEM); Transmission electron microscopy (TEM)

From: A novel antimicrobial peptide screened by a Bacillus subtilis expression system, derived from Larimichthys crocea Ferritin H, exerting bactericidal and parasiticidal activities

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● Scanning electron microscope (SEM); NPN uptake assay; PI uptake assay

From: Molecular characterization and antimicrobial activity of cecropin family in Hermetia illucens

● PI uptake assay

From: Cell-free biosynthesis combined with deep learning accelerates de novo-development of antimicrobial peptides

● PI uptake assay

From: Cell-free biosynthesis combined with deep learning accelerates de novo-development of antimicrobial peptides

● PI uptake assay

From: Cell-free biosynthesis combined with deep learning accelerates de novo-development of antimicrobial peptides

● PI uptake assay

From: Cell-free biosynthesis combined with deep learning accelerates de novo-development of antimicrobial peptides

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● PI uptake assay

From: Cell-free biosynthesis combined with deep learning accelerates de novo-development of antimicrobial peptides

● NPN uptake assay; DiSC3(5) uptake assay; PI uptake assay

From: In Vitro Evaluation of Antimicrobial Peptides from the Black Soldier Fly (Hermetia Illucens) against a Selection of Human Pathogens

● NPN uptake assay; DiSC3(5) uptake assay

From: Peptides from non-immune proteins target infections through antimicrobial and immunomodulatory properties

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Peptides from non-immune proteins target infections through antimicrobial and immunomodulatory properties

● Electrophysiological technique

From: Optimizing Antimicrobial Peptide Design: Integration of Cell-Penetrating Peptides, Amyloidogenic Fragments, and Amino Acid Residue Modifications

● NPN uptake assay; DiSC3(5) uptake assay; PI uptake assay

From: In Vitro Evaluation of Antimicrobial Peptides from the Black Soldier Fly (Hermetia Illucens) against a Selection of Human Pathogens

● PI uptake assay

From: Cell-free biosynthesis combined with deep learning accelerates de novo-development of antimicrobial peptides

● Intracellular proteins leakage assay

From: Synthesis, antimicrobial activity, and mechanistic studies of enterocin DD14, a leaderless two-peptide bacteriocin

● NPN uptake assay; DiSC3(5) uptake assay

From: Peptides from non-immune proteins target infections through antimicrobial and immunomodulatory properties

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● Intracellular proteins leakage assay

From: Synthesis, antimicrobial activity, and mechanistic studies of enterocin DD14, a leaderless two-peptide bacteriocin

● NPN uptake assay; DiSC3(5) uptake assay

From: Peptides from non-immune proteins target infections through antimicrobial and immunomodulatory properties

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● Fluorescence microscopy (FM)

From: Screening and Functional Analyses of Novel Cecropins from Insect Transcriptome

● NPN uptake assay; DiSC3(5) uptake assay

From: Peptides from non-immune proteins target infections through antimicrobial and immunomodulatory properties

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● Confocal laser scanning microscopy (CLSM); PI uptake assay; DiSC3(5) uptake assay

From: Antimicrobial Peptide Cec4 Eradicates Multidrug-Resistant Acinetobacter baumannii in vitro and in vivo

● Lactate dehydrogenase (LDH) release assay

From: Structure–activity relationships of the intramolecular disulphide bonds in LEAP2, an antimicrobial peptide from Acrossocheilus fasciatus

● NPN uptake assay; DiSC3(5) uptake assay

From: Peptides from non-immune proteins target infections through antimicrobial and immunomodulatory properties

● NPN uptake assay; DiSC3(5) uptake assay

From: Peptides from non-immune proteins target infections through antimicrobial and immunomodulatory properties

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● ONPG assay; Flow cytometry

From: Acidocin A and Acidocin 8912 Belong to a Distinct Subfamily of Class II Bacteriocins with a Broad Spectrum of Antimicrobial Activity

● NPN uptake assay; DiSC3(5) uptake assay

From: Peptides from non-immune proteins target infections through antimicrobial and immunomodulatory properties

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● Transmission electron microscopy (TEM); Scanning electron microscope (SEM); ONPG assay

From: The antimicrobial effect of a novel peptide LL-1 on Escherichia coli by increasing membrane permeability

● SYTOX Green uptake assay; NPN uptake assay

From: PaP1, a Broad-Spectrum Lysin-Derived Cationic Peptide to Treat Polymicrobial Skin Infections

● NPN uptake assay; DiSC3(5) uptake assay

From: Peptides from non-immune proteins target infections through antimicrobial and immunomodulatory properties

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● Liposome leakage assay; Atomic force microscopy (AFM); Scanning electron microscope (SEM)

From: Transition between Different Diffusion Modes of Individual Lipids during the Membrane-Specific Action of As-CATH4 Peptides

● Membrane permeability assay

From: PACAP sequence modifications modulate the peptide antimicrobial activity against bacterial pathogens affecting aquaculture

● Membrane permeability assay

From: PACAP sequence modifications modulate the peptide antimicrobial activity against bacterial pathogens affecting aquaculture

● Alkaline phosphatase (ALP) leakage assay; PI uptake assay; NPN uptake assay; DiSC3(5) uptake assay

From: Identification of antimicrobial peptides from the human gut microbiome using deep learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● DIBAC4(3) uptake assay; Flow cytometry

From: Antimicrobial peptide LL-37 disrupts plasma membrane and calcium homeostasis in Candida albicans via the Rim101 pathway

● Scanning electron microscope (SEM)

From: Botryllin, a Novel Antimicrobial Peptide from the Colonial AscidianBotryllus schlosseri

● Fluorescence microscopy (FM); Scanning electron microscope (SEM)

From: Antibacterial peptide PMAP-37(F34-R), expressed in Pichia pastoris, is effective against pathogenic bacteria and preserves plums

● Fluorescence microscopy (FM)

From: Screening and Functional Analyses of Novel Cecropins from Insect Transcriptome

● Transmission electron microscopy (TEM); Scanning electron microscope (SEM); Flow cytometry; NPN uptake assay; DiSC3(5) uptake assay

From: Antibiofilm property and multiple action of peptide PEW300 against Pseudomonas aeruginosa

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● Alkaline phosphatase (ALP) leakage assay; Fluorescence microscopy (FM)

From: Antimicrobial peptide WAM-1: a promising antibacterial and anti-inflammatory drug against carbapenem-resistant Klebsiella pneumoniae

● Scanning electron microscope (SEM); Transmission electron microscopy (TEM)

From: A novel antimicrobial peptide derived from human BPIFA1 protein protects against Candida albicans infection

● Fluorescence microscopy (FM)

From: Screening and Functional Analyses of Novel Cecropins from Insect Transcriptome

● Alkaline phosphatase (ALP) leakage assay; PI uptake assay; NPN uptake assay; DiSC3(5) uptake assay

From: Identification of antimicrobial peptides from the human gut microbiome using deep learning

● Alkaline phosphatase (ALP) leakage assay; PI uptake assay; NPN uptake assay; DiSC3(5) uptake assay

From: Identification of antimicrobial peptides from the human gut microbiome using deep learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Peptides from non-immune proteins target infections through antimicrobial and immunomodulatory properties

● Transmission electron microscopy (TEM); Alkaline phosphatase(ALP) leakage assay; PI uptake assay; NPN uptake assay; DiSC3(5) uptake assay

From: Identification of antimicrobial peptides from the human gut microbiome using deep learning

● Alkaline phosphatase (ALP) leakage assay; PI uptake assay; NPN uptake assay; DiSC3(5) uptake assay

From: Identification of antimicrobial peptides from the human gut microbiome using deep learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● Calcein leakage assay; PI uptake assay

From: Functional Analyses of Three Targeted DNA Antimicrobial Peptides Derived from Goats

● Scanning electron microscope (SEM); PI uptake assay; NPN uptake assay

From: PAM-1: an antimicrobial peptide with promise against ceftazidime-avibactam resistant Escherichia coli infection

● Alkaline phosphatase (ALP) leakage assay; PI uptake assay; NPN uptake assay; DiSC3(5) uptake assay

From: Identification of antimicrobial peptides from the human gut microbiome using deep learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● Flow cytometry; DiSC3(5) uptake assay; Transmission electron microscopy (TEM); Confocal laser scanning microscopy (CLSM)

From: Rapid Membrane-Penetrating Hybrid Peptides Achieve Efficient Dual Antimicrobial and Antibiofilm Activity through a Triple Bactericidal Mechanism

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● PI uptake assay; ONPG assay; NPN uptake assay; DiSC3(5) uptake assay; Transmission electron microscopy (TEM)

From: A novel cathelicidin TS-CATH derived from Thamnophis sirtalis combats drug-resistant gram-negative bacteria in vitro and in vivo

● NPN uptake assay; DiSC3(5) uptake assay

From: Peptides from non-immune proteins target infections through antimicrobial and immunomodulatory properties

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● PI uptake assay; Atomic force microscopy (AFM)

From: Anisaxins, helical antimicrobial peptides from marine parasites, kill resistant bacteria by lipid extraction and membrane disruption

● PI uptake assay; Atomic force microscopy (AFM)

From: Anisaxins, helical antimicrobial peptides from marine parasites, kill resistant bacteria by lipid extraction and membrane disruption

● Scanning electron microscope (SEM); NPN uptake assay; DiSC3(5) uptake assay

From: Conformation Dependent Architectures of Assembled Antimicrobial Peptides with Enhanced Antimicrobial Ability

● Liposome leakage assay

From: Modified CLEC3A-Derived Antimicrobial Peptides Lead to Enhanced Antimicrobial Activity against Drug-Resistant Bacteria

● NPN uptake assay; DiSC3(5) uptake assay

From: Peptides from non-immune proteins target infections through antimicrobial and immunomodulatory properties

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay; Scanning electron microscope (SEM)

From: Identifying novel antimicrobial peptides from venom gland of spider Pardosa astrigera by deep multi-task learning

● Fluorescence microscopy (FM)

From: Rational design of bifunctional chimeric peptides that combine antimicrobial and titanium binding activity

● Fluorescence microscopy (FM)

From: Rational design of bifunctional chimeric peptides that combine antimicrobial and titanium binding activity

● Fluorescence microscopy (FM)

From: Rational design of bifunctional chimeric peptides that combine antimicrobial and titanium binding activity

● Fluorescence microscopy (FM); Scanning electron microscope (SEM); Transmission electron microscopy (TEM)

From: Study of the Antimicrobial Activity of the Human Peptide SQQ30 against Pathogenic Bacteria

● Fluorescence microscopy (FM); DiSC3(5) uptake assay

From: A Novel Cryptic Clostridial Peptide That Kills Bacteria by a Cell Membrane Permeabilization Mechanism

● Alkaline phosphatase (ALP) leakage assay; PI uptake assay; NPN uptake assay; DiSC3(5) uptake assay

From: Identification of antimicrobial peptides from the human gut microbiome using deep learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay

From: Monomodular Pseudomonas aeruginosa phage JG004 lysozyme (Pae87) contains a bacterial surface-active antimicrobial peptide-like region and a possible substrate-binding subdomain

● Scanning electron microscope (SEM)

From: Purification and Biological Properties of Raniseptins-3 and -6, Two Antimicrobial Peptides from Boana raniceps (Cope, 1862) Skin Secretion

● DiSC3(5) uptake assay

From: Molecular characterization of the PhiKo endolysin from Thermus thermophilus HB27 bacteriophage phiKo and its cryptic lytic peptide RAP-29

● Flow cytometry; DiSC3(5) uptake assay; Transmission electron microscopy (TEM); Confocal laser scanning microscopy (CLSM)

From: Rapid Membrane-Penetrating Hybrid Peptides Achieve Efficient Dual Antimicrobial and Antibiofilm Activity through a Triple Bactericidal Mechanism

● Fluorescence microscopy (FM); Transmission electron microscopy (TEM)

From: Exploring Antimicrobial Peptides Efficacy against Fire Blight (Erwinia amylovora)

● NPN uptake assay; DiSC3(5) uptake assay

From: Molecular de-extinction of ancient antimicrobial peptides enabled by machine learning

● Alkaline phosphatase (ALP) leakage assay; PI uptake assay; NPN uptake assay; DiSC3(5) uptake assay

From: Identification of antimicrobial peptides from the human gut microbiome using deep learning

● Transmission electron microscopy (TEM)

From: Chicken cathelicidins as potent intrinsically disordered biocides with antimicrobial activity against infectious pathogens

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● Transmission electron microscopy (TEM)

From: Potential therapeutic use of dermaseptin S4 from the frog Phyllomedusa sauvagii and its derivatives against bacterial pathogens in fish

● Scanning electron microscope (SEM)

From: Purification and Biological Properties of Raniseptins-3 and -6, Two Antimicrobial Peptides from Boana raniceps (Cope, 1862) Skin Secretion

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● Scanning electron microscope (SEM); Transmission electron microscopy (TEM); PI uptake assay

From: Insight into the inhibitory activity and mechanism of bovine cathelicidin BMAP 27 against Salmonella Typhimurium

● Scanning electron microscope (SEM); Transmission electron microscopy (TEM)

From: Antibacterial and anticancer activity of two NK-lysin-derived peptides from the Antarctic teleost Trematomus bernacchii

● Liposome leakage assay

From: Anti-Protozoan Activities of Polar Fish-Derived Polyalanine Synthetic Peptides

● Liposome leakage assay

From: H1 helix of colicin U causes phospholipid membrane permeation

● Liposome leakage assay

From: An evidence of pores in phospholipid membrane induced by an antimicrobial peptide NK-2

● Calcein leakage assay

From: Molecular and energetic analysis of the interaction and specificity of Maximin 3 with lipid membranes: In vitro and in silico assessments

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● Scanning electron microscope (SEM); Transmission electron microscopy (TEM); Fluorescence microscopy (FM); SYTOX Green uptake assay

From: Antimicrobial peptide A20L: in vitro and in vivo antibacterial and antibiofilm activity against carbapenem-resistant Klebsiella pneumoniae

● Scanning electron microscope (SEM); NPN uptake assay; DiSC3(5) uptake assay

From: Conformation Dependent Architectures of Assembled Antimicrobial Peptides with Enhanced Antimicrobial Ability

● Scanning electron microscope (SEM)

From: Antimicrobial and Anti-Inflammatory Activities of MAF-1-Derived Antimicrobial Peptide Mt6 and Its D-Enantiomer D-Mt6 against Acinetobacter baumannii by Targeting Cell Membranes and Lipopolysaccharide Interaction

● Atomic force microscopy (AFM)

From: Truncated analog Brevinin2-CE-N26V5K: Revelation the Augmentation of Antimicrobial Activity

● Fluorescence microscopy (FM)

From: New Antimicrobial Peptide with Two CRAC Motifs: Activity against Escherichia coli and Bacillus subtilis

● Flow cytometry; Scanning electron microscope (SEM)

From: Novel Antimicrobial Peptide “Octoprohibitin” against Multidrug Resistant Acinetobacter baumannii

● Liposome leakage assay

From: Membranolytic Mechanism of Amphiphilic Antimicrobial β-Stranded [KL]n Peptides

● Transmission electron microscopy (TEM)

From: Chicken cathelicidins as potent intrinsically disordered biocides with antimicrobial activity against infectious pathogens.

● Liposome leakage assay

From: Membrane Interactions of Latarcins: Antimicrobial Peptides from Spider Venom

● Flow cytometry; PI uptake assay

From: Antagonistic activity of Lactobacillus plantarum C11: two new two-peptide bacteriocins, plantaricins EF and JK, and the induction factor plantaricin A

● SYTOX Green uptake assay; ATP leakage assay

From: Selective membrane disruption: mode of action of C16G2, a specifically targeted antimicrobial peptide

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● DIBAC4(3) uptake assay; Transmission electron microscopy (TEM); Scanning electron microscope (SEM)

From: Optimization of sequence and chiral content enhances therapeutic potential of tilapia piscidin peptides

● DIBAC4(3) uptake assay; NPN uptake assay; PI uptake assay; Scanning electron microscope (SEM); Transmission electron microscopy (TEM)

From: Sequential rearrangement and stereochemical reorganization to design an antimicrobial peptide with enhanced stability

● Scanning electron microscope (SEM); NPN uptake assay; DiSC3(5) uptake assay

From: Conformation Dependent Architectures of Assembled Antimicrobial Peptides with Enhanced Antimicrobial Ability

● Scanning electron microscope (SEM)

From: LcCCL28-25, Derived from Piscine Chemokine, Exhibits Antimicrobial Activity against Gram-Negative and Gram-Positive Bacteria In Vitro and In Vivo

● Electrical bilayer recording technique

From: Characterisation of cell membrane interaction mechanisms of antimicrobial peptides by electrical bilayer recording

● DIBAC4(3) uptake assay uptake assay; PI; SYTOX Green uptake assay

From: Exploring the Therapeutic Potential of Scorpion-Derived Css54 Peptide Against Candida albicans

● Liposome leakage assay

From: Membrane Interactions of Latarcins: Antimicrobial Peptides from Spider Venom

● Carboxyfluorescein leakage assay

From: Solution structure of termite-derived antimicrobial peptide, spinigerin, as determined in SDS micelle by NMR spectroscopy

● Scanning electron microscope (SEM); Transmission electron microscopy (TEM); PI uptake assay; DiSC3(5) uptake assay

From: Anti-Candida activity of two-peptide bacteriocins, plantaricins (Pln E/F and J/K) and their mode of action

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● Calcein leakage assay

From: Combating fungal phytopathogens with human salivary antimicrobial peptide histatin 5 through a multi-target mechanism

● ONPG assay; Flow cytometry

From: Acidocin A and Acidocin 8912 Belong to a Distinct Subfamily of Class II Bacteriocins with a Broad Spectrum of Antimicrobial Activity

● Liposome leakage assay; Flow cytometry

From: Where Electrostatics Matter: Bacterial Surface Neutralization and Membrane Disruption by Antimicrobial Peptides SAAP-148 and OP-145

● Liposome leakage assay; Flow cytometry

From: Where Electrostatics Matter: Bacterial Surface Neutralization and Membrane Disruption by Antimicrobial Peptides SAAP-148 and OP-145

● Patch-Clamp electrophysiology

From: Mechanistic Insight into the Early Stages of Toroidal Pore Formation by the Antimicrobial Peptide Smp24

● Scanning electron microscope (SEM)

From: Antimicrobial potential of a ponericin-like peptide isolated from Bombyx mori L. hemolymph in response to Pseudomonas aeruginosa infection

● PI uptake assay; NPN uptake assay; Scanning electronic microscopy (SEM)

From: Rational design of Abhisin-like peptides enables generation of potent antimicrobial activity against pathogens

● NPN uptake assay; DiSC3(5) uptake assay

From: Molecular de-extinction of ancient antimicrobial peptides enabled by machine learning

● SYTOX Green uptake assay; DiSC3(5) uptake assay

From: A plausible mode of action of pseudin-2, an antimicrobial peptide from Pseudis paradoxa

● Transmission electron microscopy (TEM)

From: Characterization of two linear cationic antimalarial peptides in the scorpion Mesobuthus eupeus

● Scanning electron microscope (SEM); DiSC3(5) uptake assay; Lactate dehydrogenase (LDH) release assay

From: In Silico-Based Design of a Hybrid Peptide with Antimicrobial Activity against Multidrug-Resistant Pseudomonas aeruginosa Using a Spider Toxin Peptide

● Fluorescence microscopy (FM); Scanning electron microscope (SEM)

From: Molecular characterization, antibacterial activity and mechanism analyzation of three different piscidins from black rockfish, Sebastes schlegelii

● Scanning electron microscope (SEM); NPN uptake assay; PI uptake assay

From: Octominin: An antimicrobial peptide with antibacterial and anti-inflammatory activity against carbapenem-resistant Escherichia coli both in vitro and in vivo

● Membrane binding assay

From: Development of Antimicrobial Peptide-Antibiotic Conjugates to Improve the Outer Membrane Permeability of Antibiotics Against Gram-Negative Bacteria

● ONPG assay; DiSC3(5) uptake assay

From: A Novel Antimicrobial Peptide, Dermaseptin-SS1, with Anti-Proliferative Activity, Isolated from the Skin Secretion of Phyllomedusa tarsius

● Calcein leakage assay

From: Role of interfacial hydrophobicity in antimicrobial peptide magainin 2-induced nanopore formation

● Scanning electron microscope (SEM)

From: Broad-spectrum hybrid antimicrobial peptides derived from PMAP-23 with potential LPS binding ability

● Scanning electron microscope (SEM)

From: Broad-spectrum hybrid antimicrobial peptides derived from PMAP-23 with potential LPS binding ability

● Fluorescence microscopy (FM)

From: Oreoch-1: A Peptide from Oreochromis niloticus as a Potential Tool against Staphylococci

● Alkaline phosphatase (ALP) leakage assay; PI uptake assay; NPN uptake assay; DiSC3(5) uptake assay

From: Identification of antimicrobial peptides from the human gut microbiome using deep learning

● NMR

From: Increases in Hydrophilicity and Charge on the Polar Face of Alyteserin 1c Helix Change its Selectivity towards Gram-Positive Bacteria

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● Scanning electron microscope (SEM); NPN uptake assay

From: A Novel Antimicrobial Peptide Sp-LECin with Broad-Spectrum Antimicrobial Activity and Anti- Pseudomonas aeruginosa Infection in Zebrafish

● Fluorescence microscopy (FM)

From: Characterization of an antimicrobial peptide family from the venom gland of Heteropoda venatoria

● DIBAC4(3) uptake assay; PI uptake assay; Scanning electron microscope (SEM)

From: Development of Bactericidal Peptides against Multidrug-Resistant Acinetobacter baumannii with Enhanced Stability and Low Toxicity

● Transmission electron microscopy (TEM); Scanning electron microscope (SEM); SYTOX Green uptake assay

From: Identification and Characterization of RK22, a Novel Antimicrobial Peptide from Hirudinaria manillensis against Methicillin Resistant Staphylococcus aureus

● Confocal laser scanning microscopy (CLSM); Transmission electron microscopy (TEM); DiSC3(5) uptake assay

From: Novel antimicrobial peptide DvAMP serves as a promising antifungal agent against Cryptococcus neoformans

● NPN uptake assay; Flow cytometric analysis; Dye leakage assay

From: Systematic comparison of activity and mechanism of antimicrobial peptides against nosocomial pathogens

● Fluorescence microscopy (FM); Scanning electron microscope (SEM); ONPG assay; Alkaline phosphatase(ALP) leakage

From: An optimized antimicrobial peptide analog acts as an antibiotic adjuvant to reverse methicillin-resistant Staphylococcus aureus

● DiSC3(5) uptake assay; Ethidium bromide assay

From: GATR-3, a Peptide That Eradicates Preformed Biofilms of Multidrug-Resistant Acinetobacter baumannii

● DiSC3(5) uptake assay; Calcein leakage assay

From: Host Defense Peptide Piscidin and Yeast-Derived Glycolipid Exhibit Synergistic Antimicrobial Action through Concerted Interactions with Membranes

● Scanning electron microscope (SEM)

From: Trematocine-derived antimicrobial peptides from the Antarctic fish Trematomus bernacchaii: potent antibacterial agents against ESKAPE pathogens

● Confocal laser scanning microscopy (CLSM)

From: Interaction of Pexiganan (MSI-78)-Derived Analogues Reduces Inflammation and TLR4-Mediated Cytokine Secretion: A Comparative Study

● NPN uptake assay; Fluorescence microscopy (FM)

From: Exploring the active core of a novel antimicrobial peptide, palustrin-2LTb, from the Kuatun frog, Hylarana latouchii, using a bioinformatics-directed approach

● SYTOX Green uptake assay; NPN uptake assay; Fluorescence microscopy (FM)

From: Targeted Modification and Structure-Activity Study of GL-29, an Analogue of the Antimicrobial Peptide Palustrin-2ISb

● Scanning electron microscope (SEM); Flow cytometry; Confocal laser scanning microscopy (CLSM)

From: The antibacterial activity and mechanism of a novel peptide MR-22 against multidrug-resistant Escherichia coli

● SYTOX Green uptake assay

From: Progressive Design of a Ranatuerin-2 Peptide from Amolops wuyiensis: Enhancement of Bioactivity and In Vivo Efficacy

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● Transmission electron microscopy (TEM)

From: Preliminary investigation of the immune activity of PmH2A-derived antimicrobial peptides from the pearl oyster Pinctada fucata martensii

● Transmission electron microscopy (TEM)

From: Preliminary investigation of the immune activity of PmH2A-derived antimicrobial peptides from the pearl oyster Pinctada fucata martensii

● Fluorescence microscopy (FM)

From: Molecular characterization of histone gene in golden pompano (Trachinotus ovatus) and antimicrobial activity of its derived peptides

● PI uptake assay; Transmission electron microscopy (TEM)

From: In silico identification of two peptides with antibacterial activity against multidrug-resistant Staphylococcus aureus

● DiSC3(5) uptake assay; Transmission electron microscopy (TEM)

From: Optimizing Bothropstoxin-I-Derived Peptides: Exploring the Antibacterial Potential of p-BthW

● DiSC3(5) uptake assay; PI uptake assay

From: Novel Alligator Cathelicidin As-CATH8 Demonstrates Anti-Infective Activity against Clinically Relevant and Crocodylian Bacterial Pathogens

● SYTOX Green uptake assay

From: Strategic Single-Residue Substitution in the Antimicrobial Peptide Esc(1–21) Confers Activity against Staphylococcus aureus, Including Drug-Resistant and Biofilm Phenotype

● Electrical bilayer recording technique

From: Characterisation of cell membrane interaction mechanisms of antimicrobial peptides by electrical bilayer recording

● Liposome leakage assay

From: Effect of Phosphatidylethanolamine on Pore Formation Induced by the Antimicrobial Peptide PGLa

● Scanning electron microscope (SEM); NPN uptake assay; Transmission electron microscopy (TEM)

From: Unlocking Antibacterial Potential: Key-Site-Based Regulation of Antibacterial Spectrum of Peptides

● PI uptake assay

From: The effects of magainin 2-derived and rationally designed antimicrobial peptides on Mycoplasma pneumoniae

● PI uptake assay

From: The effects of magainin 2-derived and rationally designed antimicrobial peptides on Mycoplasma pneumoniae

● PI uptake assay

From: The effects of magainin 2-derived and rationally designed antimicrobial peptides on Mycoplasma pneumoniae

● Transmission electron microscopy (TEM)

From: Dual antibacterial mechanism of [K4K15]CZS-1 against Salmonella Typhimurium: a membrane active and intracellular-targeting antimicrobial peptide

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● Liposome leakage assay

From: The C-terminus of the GKY20 antimicrobial peptide, derived from human thrombin, plays a key role in its membrane perturbation capability

● Liposome leakage assay

From: Identification of Bacterial Membrane Selectivity of Romo1-Derived Antimicrobial Peptide AMPR-22 via Molecular Dynamics

● Fluorescence microscopy (FM); Scanning electron microscope (SEM)

From: Molecular characterization, antibacterial activity and mechanism analyzation of three different piscidins from black rockfish, Sebastes schlegelii

● SYTOX Green uptake assay

From: Antimicrobial Spectrum of Activity and Mechanism of Action of Linear Alpha-Helical Peptides Inspired by Shrimp Anti-Lipopolysaccharide Factors

● SYTOX Green uptake assay

From: Antimicrobial Spectrum of Activity and Mechanism of Action of Linear Alpha-Helical Peptides Inspired by Shrimp Anti-Lipopolysaccharide Factors

● NPN uptake assay; Scanning electron microscope (SEM)

From: Designing a Novel Functional Peptide With Dual Antimicrobial and Anti-inflammatory Activities via in Silico Methods

● NPN uptake assay; Scanning electron microscope (SEM)

From: Designing a Novel Functional Peptide With Dual Antimicrobial and Anti-inflammatory Activities via in Silico Methods

● Scanning electron microscope (SEM); SYTOX Green uptake assay

From: A Novel Antimicrobial Peptide Derived from Bony Fish IFN1 Exerts Potent Antimicrobial and Anti-Inflammatory Activity in Mammals

● Fluorescence microscopy (FM)

From: Molecular characterization of histone gene in golden pompano (Trachinotus ovatus) and antimicrobial activity of its derived peptides

● ONPG assay; PI uptake assay

From: Characterization of novel antimicrobial peptides designed on the basis of amino acid sequence of peptides from egg white hydrolysate

● NPN uptake assay; DiSC3(5) uptake assay; Scanning electron microscope (SEM)

From: Identifying novel antimicrobial peptides from venom gland of spider Pardosa astrigera by deep multi-task learning

● Scanning electron microscope (SEM)

From: Cecropin D-derived synthetic peptides in the fight against Candida albicans cell filamentation and biofilm formation

● Scanning electron microscope (SEM)

From: Cecropin D-derived synthetic peptides in the fight against Candida albicans cell filamentation and biofilm formation

● ONPG assay; Transmission electron microscopy (TEM)

From: Antibacterial Activity and Mechanism of Peptide PV-Q5 against Vibrio parahaemolyticus and Escherichia coli, Derived from Salt-Fermented Penaeus vannamei

● Confocal laser scanning microscopy (CLSM); Scanning electron microscope (SEM)

From: Legume Plant Peptides as Sources of Novel Antimicrobial Molecules Against Human Pathogens

● Flow cytometry; Scanning electron microscope (SEM); NPN uptake assay

From: Lysine-Tethered Stable Bicyclic Cationic Antimicrobial Peptide Combats Bacterial Infection in Vivo

● PI uptake assay; Transmission electron microscopy (TEM)

From: Microbiome-derived antimicrobial peptides offer therapeutic solutions for the treatment of Pseudomonas aeruginosa infections

● PI uptake assay; Transmission electron microscopy (TEM)

From: Microbiome-derived antimicrobial peptides show therapeutic activity against the critically important priority pathogen, Acinetobacter baumannii

● PI uptake assay; Transmission electron microscopy (TEM)

From: Microbiome-derived antimicrobial peptides offer therapeutic solutions for the treatment of Pseudomonas aeruginosa infections

● DiSC3(5) uptake assay; Fluorescence microscopy (FM)

From: Phage-displayed heptapeptide sequence conjugation significantly improves the specific targeting ability of antimicrobial peptides against Staphylococcus aureus

● NPN uptake assay; DiSC3(5) uptake assay

From: Molecular de-extinction of ancient antimicrobial peptides enabled by machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: Discovery of antimicrobial peptides in the global microbiome with machine learning

● Fluorescence microscopy (FM); Scanning electron microscope (SEM)

From: Molecular characterization, antibacterial activity and mechanism analyzation of three different piscidins from black rockfish, Sebastes schlegelii

● SYTOX Green uptake assay; PI uptake assay

From: Cationicity Enhancement on the Hydrophilic Face of Ctriporin Significantly Reduces Its Hemolytic Activity and Improves the Antimicrobial Activity against Antibiotic-Resistant ESKAPE Pathogens

● Liposome leakage assay

From: Membrane fluidity, composition, and charge affect the activity and selectivity of the AMP ascaphin-8

● Liposome leakage assay

From: Membrane fluidity, composition, and charge affect the activity and selectivity of the AMP ascaphin-8

● Liposome leakage assay

From: Membrane fluidity, composition, and charge affect the activity and selectivity of the AMP ascaphin-8

● Liposome leakage assay

From: Membrane fluidity, composition, and charge affect the activity and selectivity of the AMP ascaphin-8

● Liposome leakage assay

From: Antifungal activity and mode of action of synthetic peptides derived from the tick OsDef2 defensin

● NPN uptake assay; Flow cytometric analysis; Dye leakage assay

From: Systematic comparison of activity and mechanism of antimicrobial peptides against nosocomial pathogens

● Scanning electron microscope (SEM)

From: Antibacterial and Antifungal Properties of a Novel Antimicrobial Peptide GK-19 and Its Application in Skin and Soft Tissue Infections Induced by MRSA or Candida albicans

● Scanning electron microscope (SEM); SYTOX Green uptake assay

From: LC-AMP-F1 Derived from the Venom of the Wolf Spider Lycosa coelestis, Exhibits Antimicrobial and Antibiofilm Activities

● Scanning electron microscope (SEM)

From: Antibacterial Potential Analysis of Novel α-Helix Peptides in the Chinese Wolf Spider Lycosa sinensis

● Transmission electron microscopy (TEM); Confocal laser scanning microscopy (CLSM); Flow cytometry; ONPG assay

From: Antibacterial Peptide NP-6 Affects Staphylococcus aureus by Multiple Modes of Action

● Scanning electron microscope (SEM); Transmission electron microscopy (TEM); NPN uptake assay; DiSC3(5) uptake assay

From: pH-Responsive Antimicrobial Peptide with Selective Killing Activity for Bacterial Abscess Therapy

● Scanning electron microscope (SEM); Transmission electron microscopy (TEM); NPN uptake assay; DiSC3(5) uptake assay

From: pH-Responsive Antimicrobial Peptide with Selective Killing Activity for Bacterial Abscess Therapy

● DiSC3(5) uptake assay; Liposome leakage assay

From: A novel hybrid peptide composed of LfcinB6 and KR-12-a4 with enhanced antimicrobial, anti-inflammatory and anti-biofilm activities

● PI uptake assay; Transmission electron microscopy (TEM)

From: Microbiome-derived antimicrobial peptides offer therapeutic solutions for the treatment of Pseudomonas aeruginosa infections

● Confocal laser scanning microscopy (CLSM); NPN uptake assay; Scanning electron microscope (SEM); Transmission electron microscopy (TEM); ONPG assay; DiSC3(5) uptake assay

From: Development of Neuropeptide Hemokinin-1 Analogues with Antimicrobial and Wound-Healing Activity

● SYTOX Green uptake assay

From: Discovery and engineering of a novel peptide, Temporin-WY2, with enhanced in vitro and in vivo efficacy against multi-drug resistant bacteria

● SYTOX Green uptake assay

From: Discovery and engineering of a novel peptide, Temporin-WY2, with enhanced in vitro and in vivo efficacy against multi-drug resistant bacteria

● SYTOX Green uptake assay; NPN uptake assay

From: Enhancement of Antimicrobial Function by L/D-Lysine Substitution on a Novel Broad-Spectrum Antimicrobial Peptide, Phylloseptin-TO2: A Structure-Related Activity Research Study

● SYTOX Green uptake assay; NPN uptake assay

From: Enhancement of Antimicrobial Function by L/D-Lysine Substitution on a Novel Broad-Spectrum Antimicrobial Peptide, Phylloseptin-TO2: A Structure-Related Activity Research Study

● Scanning electron microscope (SEM); DiSC3(5) uptake assay; Lactate dehydrogenase (LDH) release assay

From: In Silico-Based Design of a Hybrid Peptide with Antimicrobial Activity against Multidrug-Resistant Pseudomonas aeruginosa Using a Spider Toxin Peptide

● SYTOX Green uptake assay; Confocal laser scanning microscopy (CLSM); DiSC3(5) uptake assay

From: Discovery of structural and functional transition sites for membrane-penetrating activity of sheep myeloid antimicrobial peptide-18

● NPN uptake assay; DIBAC4(3) uptake assay; Scanning electron microscope (SEM); Transmission electron microscopy (TEM)

From: Strategic modification of low-activity natural antimicrobial peptides confers antibacterial potential in vitro and in vivo

● Fluorescence microscopy (FM); Scanning electron microscope (SEM)

From: A new bioinspired peptide on defensin from C. annuum fruits: Antimicrobial activity, mechanisms of action and therapeutical potential

● Calcein leakage assay

From: A rationally designed synthetic antimicrobial peptide against Pseudomonas-associated corneal keratitis: Structure-function correlation

● SYTOX Green uptake assay

From: Multifunctional Properties of BMAP-18 and Its Aliphatic Analog against Drug-Resistant Bacteria

● Scanning electron microscope (SEM)

From: Antibacterial Potential Analysis of Novel α-Helix Peptides in the Chinese Wolf Spider Lycosa sinensis

● Transmission electron microscopy (TEM)

From: Lacticaseibacillus paracasei fermentation broth identified peptide, Y2Fr, and its antibacterial activity on Vibrio parahaemolyticus

● Scanning electron microscope (SEM)

From: Identification and Characterization of a Potential Antimicrobial Peptide Isolated from Soil Brevibacillus sp. WUL10 and Its Activity against MRSA Pathogens

● PI uptake assay; Scanning electron microscope (SEM); Transmission electron microscopy (TEM)

From: Development of α-Helical Antimicrobial Peptides with Imperfect Amphipathicity for Superior Activity and Selectivity

● NPN uptake assay; DiSC3(5) uptake assay; Confocal laser scanning microscopy (CLSM); Scanning electron microscope (SEM); PI uptake assay

From: Antibacterial efficacy and mechanism of Cyprinus carpio chemokine-derived L-10 against multidrug-resistant Escherichia coli infections

● Fluorescence microscopy (FM); Liposome leakage; NPN uptake assay; DiSC3(5) uptake assay

From: Bactericidal activities and action mechanism of the novel antimicrobial peptide Hylin a1 and its analog peptides against Acinetobacter baumannii infection

● Fluorescence microscopy (FM); Liposome leakage; NPN uptake assay; DiSC3(5) uptake assay

From: Bactericidal activities and action mechanism of the novel antimicrobial peptide Hylin a1 and its analog peptides against Acinetobacter baumannii infection

● Fluorescence microscopy (FM); Liposome leakage; NPN uptake assay; DiSC3(5) uptake assay

From: Bactericidal activities and action mechanism of the novel antimicrobial peptide Hylin a1 and its analog peptides against Acinetobacter baumannii infection

● PI uptake assay; DiSC3(5) uptake assay; Scanning electron microscope (SEM)

From: Design, biological characteristics, and antibacterial mechanism of high therapeutic index antimicrobial peptides with PRRP as central axis

● PI uptake assay; DiSC3(5) uptake assay; Scanning electron microscope (SEM)

From: Design, biological characteristics, and antibacterial mechanism of high therapeutic index antimicrobial peptides with PRRP as central axis

● Flow cytometry; Transmission electron microscopy (TEM)

From: A novel designed membrane-active peptide for the control of foodborne Salmonella enterica serovar Typhimurium

● Scanning electron microscope (SEM); Transmission electron microscopy (TEM); NPN uptake assay; DiSC3(5) uptake assay; PI uptake assay

From: The design of cell-selective tryptophan and arginine-rich antimicrobial peptides by introducing hydrophilic uncharged residues

● SYTOX Green uptake assay; Fluorescence microscopy (FM)

From: Computer-made peptide RQ18 acts as a dual antifungal and antibiofilm peptide though membrane-associated mechanisms of action

● NPN uptake assay; DiSC3(5) uptake assay; Flow cytometry; Scanning electron microscope (SEM)

From: Antibacterial, Antibiofilm, and Anti-inflammatory Effects of a Novel Thrombin-Derived Peptide in Sepsis Models: Insights into Underlying Mechanisms

● Scanning electron microscope (SEM)

From: Distinct mode of membrane interaction and disintegration by diverse class of antimicrobial peptides

● Liposome leakage assay; Scanning electron microscope (SEM)

From: A Peptide-Nanoparticle System with Improved Efficacy against Multidrug Resistant Bacteria

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● PI uptake assay

From: Hydrophobicity Determines the Bacterial Killing Rate of α-Helical Antimicrobial Peptides and Influences the Bacterial Resistance Development

● PI uptake assay

From: Hydrophobicity Determines the Bacterial Killing Rate of α-Helical Antimicrobial Peptides and Influences the Bacterial Resistance Development

● PI uptake assay

From: Hydrophobicity Determines the Bacterial Killing Rate of α-Helical Antimicrobial Peptides and Influences the Bacterial Resistance Development

● PI uptake assay

From: Hydrophobicity Determines the Bacterial Killing Rate of α-Helical Antimicrobial Peptides and Influences the Bacterial Resistance Development

● Scanning electron microscope (SEM)

From: Antimicrobial peptides designed by computational analysis of proteomes

● Transmission electron microscopy (TEM); DiSC3(5) uptake assay

From: Blap-6, a Novel Antifungal Peptide from the Chinese Medicinal Beetle Blaps rhynchopetera against Cryptococcus neoformans

● NPN uptake assay; DiSC3(5) uptake assay; Confocal laser scanning microscopy (CLSM)

From: In silico identification of novel antimicrobial peptides from the venom gland transcriptome of the spider Argiope bruennichi (Scopoli, 1772)

● Scanning electron microscope (SEM); SYTOX Green uptake assay

From: Effects of structural changes on antibacterial activity and cytotoxicity due to proline substitutions in chimeric peptide HnMc

● NPN uptake assay; DiSC3(5) uptake assay; Confocal laser scanning microscopy (CLSM); Scanning electron microscope (SEM); PI uptake assay

From: Efficacy of the bee-venom antimicrobial peptide Osmin against sensitive and carbapenem-resistant Klebsiella pneumoniae strains

● SYTOX Green uptake assay

From: Discovery of a Novel Antimicrobial Peptide, Temporin-PKE, from the Skin Secretion of Pelophylax kl. esculentus, and Evaluation of Its Structure-Activity Relationships

● SYTOX Green uptake assay

From: Discovery of a Novel Antimicrobial Peptide, Temporin-PKE, from the Skin Secretion of Pelophylax kl. esculentus, and Evaluation of Its Structure-Activity Relationships

● SYTOX Green uptake assay

From: A Novel Strategy for the Design of Aurein 1.2 Analogs with Enhanced Bioactivities by Conjunction of Cell-Penetrating Regions

● NPN uptake assay; DiSC3(5) uptake assay

From: Molecular de-extinction of ancient antimicrobial peptides enabled by machine learning

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● Electrical bilayer recording technique

From: Characterisation of cell membrane interaction mechanisms of antimicrobial peptides by electrical bilayer recording

● NPN uptake assay; DiSC3(5) uptake assay; Flow cytometry

From: A Foundation Model Identifies Broad-Spectrum Antimicrobial Peptides against Drug-Resistant Bacterial Infection

● NPN uptake assay; DiSC3(5) uptake assay; Flow cytometry

From: A Foundation Model Identifies Broad-Spectrum Antimicrobial Peptides against Drug-Resistant Bacterial Infection

● Flow cytometry

From: Comparative Properties of Helical and Linear Amphipathicity of Peptides Composed of Arginine, Tryptophan, and Valine

● Atomic force microscopy (AFM); Scanning electron microscope (SEM); NPN uptake assay; DiSC3(5) uptake assay; PI uptake assay

From: Potential Broad-Spectrum Antimicrobial, Wound Healing, and Disinfectant Cationic Peptide Crafted from Snake Venom

● Scanning electron microscope (SEM); Transmission electron microscopy (TEM); PI uptake assay; Confocal laser scanning microscopy (CLSM)

From: Chickpea-Derived Modified Antimicrobial Peptides KTA and KTR Inactivate Staphylococcus aureus via Disrupting Cell Membrane and Interfering with Peptidoglycan Synthesis

● Scanning electron microscope (SEM); Transmission electron microscopy (TEM); NPN uptake assay; PI uptake assay; Flow cytometry

From: Development of antibacterial peptides with efficient antibacterial activity, low toxicity, high membrane disruptive activity and a synergistic antibacterial effect

● Transmission electron microscopy (TEM); Flow cytometry

From: Study on antimicrobial activity of sturgeon skin mucus polypeptides (Rational Design, Self-Assembly and Application)

● Scanning electron microscope (SEM)

From: Pore-forming peptide C14R exhibits potent antifungal activity against clinical isolates of Candida albicans and Candida auris

● Scanning electron microscope (SEM); DiSC3(5) uptake assay; PI uptake assay

From: Cec4-Derived Peptide Inhibits Planktonic and Biofilm-Associated Methicillin Resistant Staphylococcus epidermidis

● Liposome leakage assay

From: H1 helix of colicin U causes phospholipid membrane permeation

● Flow cytometry

From: Comparative Properties of Helical and Linear Amphipathicity of Peptides Composed of Arginine, Tryptophan, and Valine

● Scanning electron microscope (SEM)

From: Ramosin: The First Antibacterial Peptide Identified on Bolitoglossa ramosi Colombian Salamander

● Scanning electron microscope (SEM)

From: Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant Candida albicans on Contact Lenses

● Fluorescence microscopy (FM); Scanning electron microscope (SEM)

From: In vitro and in vivo Activity of Phibilin Against Candida albicans

● PI uptake assay; Scanning electron microscope (SEM); Transmission electron microscopy (TEM)

From: Rational Design of a Potent Antimicrobial Peptide Based on the Active Region of a Gecko Cathelicidin

● NPN uptake assay; DiSC3(5) uptake assay; SYTOX Green uptake assay

From: A frog-derived antimicrobial peptide as a potential anti-biofilm agent in combating Staphylococcus aureus skin infection

● Scanning electron microscope (SEM); Transmission electron microscopy (TEM); DiSC3(5) uptake assay; Flow cytometry; NPN uptake assay; Fluorescence microscopy (FM)

From: Optimization of Antibacterial Activity in Tibetan Swine α-Helix Peptide TP by Site-Directed Mutagenesis

● Scanning electron microscope (SEM); Transmission electron microscopy (TEM); DiSC3(6) uptake assay; Flow cytometry; NPN uptake assay; Fluorescence microscopy (FM)

From: Optimization of Antibacterial Activity in Tibetan Swine α-Helix Peptide TP by Site-Directed Mutagenesis

● SYTOX Green uptake assay

From: A Novel Strategy for the Design of Aurein 1.2 Analogs with Enhanced Bioactivities by Conjunction of Cell-Penetrating Regions

● Scanning electron microscope (SEM); Confocal laser scanning microscopy (CLSM)

From: Development of Antibacterial Peptides with Membrane Disruption and Folate Pathway Inhibitory Activities against Methicillin-Resistant Staphylococcus aureus

● Scanning electron microscope (SEM); Confocal laser scanning microscopy (CLSM)

From: Development of Antibacterial Peptides with Membrane Disruption and Folate Pathway Inhibitory Activities against Methicillin-Resistant Staphylococcus aureus

● Scanning electron microscope (SEM)

From: Antimicrobial and Antibiofilm Activity of Synthetic Peptide [W7]KR12-KAEK Against Enterococcus faecalis Strains

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● Scanning electron microscope (SEM)

From: Antimicrobial peptides designed by computational analysis of proteomes

● Scanning electron microscope (SEM)

From: Antifungal activity of designed α-helical antimicrobial peptides

● Scanning electron microscope (SEM)

From: Antifungal activity of designed α-helical antimicrobial peptides

● Scanning electron microscope (SEM)

From: Antifungal activity of designed α-helical antimicrobial peptides

● Scanning electron microscope (SEM)

From: Rational Design of RN15m4 Cathelin Domain-Based Peptides from Siamese Crocodile Cathelicidin Improves Antimicrobial Activity

● Transmission electron microscopy (TEM); DiSC3(5) uptake assay

From: Ribosomal protein L17 functions as an antimicrobial protein in amphioxus

● Scanning electron microscope (SEM); Liposome leakage

From: How do terminal modifications of short designed IIKK peptide amphiphiles affect their antifungal activity and biocompatibility?

● Scanning electron microscope (SEM); Transmission electron microscopy (TEM); Confocal laser scanning microscopy (CLSM)

From: Rational design and characterization of cell-selective antimicrobial peptides based on a bioactive peptide from Crocodylus siamensis hemoglobin

● Confocal laser scanning microscopy (CLSM); Flow cytometry; Scanning electron microscope (SEM)

From: Structural Characterization, Functional Profiling, and Mechanism Study of Four Antimicrobial Peptides for Antibacterial and Anticancer Applications

● NPN uptake assay; DiSC3(5) uptake assay; ONPG assay; Scanning electron microscope (SEM); Fluorescence microscopy (FM)

From: The synergy of thanatin and cathelicidin-BF-15a3 combats Escherichia coli O157:H7

● Transmission electron microscopy (TEM); ONPG assay; Confocal laser scanning microscopy (CLSM)

From: Synergistic antibacterial effects of low-intensity ultrasound and peptide LCMHC against Staphylococcus aureus

● Scanning electron microscope (SEM); SYTOX Green uptake assay

From: Purification and Characterization of Novel Anti-MRSA Peptides Produced by Brevibacillus sp. SPR-20

● Scanning electron microscope (Scanning electron microscope (SEM)); Liposome leakage assay

From: Evolutionary and in silico guided development of novel peptide analogues for antibacterial activity against ESKAPE pathogens

● Scanning electron microscope (SEM)

From: DP1, a multifaceted synthetic peptide: Mechanism of action, activity and clinical potential

● Scanning electron microscope (SEM); DiSC3(5) uptake assay; Fluorescence microscopy (FM)

From: Antibacterial Activity and Mechanisms of TroHepc2-22, a Derived Peptide of Hepcidin2 from Golden Pompano (Trachinotus ovatus)

● NPN uptake assay; DiSC3(5) uptake assay; PI uptake assay

From: IMT-P8 potentiates Gram-positive specific antibiotics in intrinsically resistant Gram-negative bacteria

● Transmission electron microscopy (TEM)

From: Human microbiome derived synthetic antimicrobial peptides with activity against Gram-negative, Gram-positive, and antibiotic resistant bacteria

● SYTOX Green uptake assay

From: Kassporin-KS1: A Novel Pentadecapeptide from the Skin Secretion of Kassina senegalensis: Studies on the Structure-Activity Relationships of Site-Specific “Glycine-Lysine” Motif Insertions

● SYTOX Green uptake assay

From: Kassporin-KS1: A Novel Pentadecapeptide from the Skin Secretion of Kassina senegalensis: Studies on the Structure-Activity Relationships of Site-Specific “Glycine-Lysine” Motif Insertions

● SYTOX Green uptake assay

From: Kassporin-KS1: A Novel Pentadecapeptide from the Skin Secretion of Kassina senegalensis: Studies on the Structure-Activity Relationships of Site-Specific “Glycine-Lysine” Motif Insertions

● SYTOX Green uptake assay

From: Kassporin-KS1: A Novel Pentadecapeptide from the Skin Secretion of Kassina senegalensis: Studies on the Structure-Activity Relationships of Site-Specific “Glycine-Lysine” Motif Insertions

● Scanning electron microscope (SEM); Transmission electron microscopy (TEM); DiSC3(5) uptake assay; Flow cytometry

From: Antimicrobial activity of novel symmetrical antimicrobial peptides centered on a hydrophilic motif against resistant clinical isolates: in vitro and in vivo analyses

● Flow cytometry; Scanning electron microscope (SEM)

From: Functional Characterization, Antimicrobial Effects, and Potential Antibacterial Mechanisms of NpHM4, a Derived Peptide of Nautilus pompilius Hemocyanin

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● NPN uptake assay; DiSC3(5) uptake assay

From: A generative artificial intelligence approach for antibiotic optimization

● Scanning electron microscope (SEM)

From: Antimicrobial peptides designed by computational analysis of proteomes

● Scanning electron microscope (SEM)

From: Antimicrobial peptides designed by computational analysis of proteomes

● Calcein leakage assay; PI uptake assay

From: Deciphering Structure-Function Relationship Unveils Salt-Resistant Mode of Action of a Potent MRSA-Inhibiting Antimicrobial Peptide, RR14

● Confocal laser scanning microscopy (CLSM); Flow cytometry; Calcein leakage assay; Scanning electron microscope (SEM)

From: Design of Antimicrobial Peptides with Cell-Selective Activity and Membrane-Acting Mechanism against Drug-Resistant Bacteria

● Surface pressure measurement

From: Structural analysis of the peptides temporin-Ra and temporin-Rb and interactions with model membranes

● Scanning electron microscope (SEM)

From: Determination of antifungal activity and action mechanism of the modified Aurein 1.2 peptide derivatives

● Scanning electron microscope (SEM)

From: Determination of antifungal activity and action mechanism of the modified Aurein 1.2 peptide derivatives

● Transmission electron microscopy (TEM); DiSC3(5) uptake assay; PI uptake assay

From: Selective antibacterial activity of a novel lactotransferrin-derived antimicrobial peptide LF-1 against Streptococcus mutans

● Scanning electron microscope (SEM); Transmission electron microscopy (TEM)

From: A 14-amino acid cationic peptide Bolespleenin334-347 from the marine fish mudskipper Boleophthalmus pectinirostris exhibiting potent antimicrobial activity and therapeutic potential

● DiSC3(5) uptake assay; Scanning electron microscope (SEM); Calcein leakage assay

From: Unlocking roles of cationic and aromatic residues in peptide amphiphiles in treating drug-resistant gram-positive pathogens

● DiSC3(5) uptake assay; Scanning electron microscope (SEM); Calcein leakage assay

From: Unlocking roles of cationic and aromatic residues in peptide amphiphiles in treating drug-resistant gram-positive pathogens